Avaliação da atividade esquistossomicida in vitro da oleorresina de Copaifera duckei e dos seus principais metabólitos

Abstract

According to the World Health Organization, schistosomiasis affects over 207 million people worldwide. In Brazil, the endemic area covers 19 states with approximately 42 million people at risk of infection. Of this total, about 7 million people are currently infected, making this disease one of the most serious health problems in developing countries. Of all the medicinal plants commonly used by the Brazilian population, the trees of the genus Copaifera, known popularly as "copaiba, copaibeiras or stick d'óleo" can be highlighted due to their pharmacological applications historically proven by popular medicine, such as antiinflammatory , antiparasitic and others. In order to exploit this potential, in this paper are described the isolation procedures and identification of metabolites found in the fixed part of balm C. duckei, in addition to trials with the oleoresin and the main metabolites isolated this species, with main emphasis on diterpenes acids (constituents of fixed fraction of the oleoresin), against Schistosoma mansoni. The results demonstrate that the oleoresin has a lethal concentration values 50% of parasites (LC50) of 75.8, 50.6 and 47.2 µg/mL after 24, 48 and 72 hours incubation respectively, and its major metabolite the poliáltico acid (Cd1) has LC50 values of 133.4 μM (41,7μg/ml), 116.3 µM (36.2 μg/ml) and 107.9 µM (33.4 μg/ml), 24, 48 and 72 hours of incubation respectively. Other aspects analyzed such as oviposition, egg development and activity against young worms denote the potential of this study to find new schistosomicide principles.